QWALYS® 3 EVO
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Antibody identification with I-Mag Ident

Identification with I-Mag Ident

The identification test for unexpected antibodies using E.M.^® Technology is based on the magnetisation of red blood cells. The principle of the method uses the indirect antiglobulin test, in solid phase, combined with a magnetic field.

• The plasma or serum of the test subject is incubated with the Group O magnetised red blood cells and the known phenotype “I-Mag Ident”. These red blood cells are sensitised in the presence of the antibody directed against an antigen on their surface.
• Once subjected to a magnetic field, all the magnetised red blood cells migrate to the bottom of the well.
• The monoclonal antiglobulins present in the ScreenLys microplate wells show the presence of immunoglobulins of IgG type attached to the red blood cells. This in turn, leads to formation of a layer of red blood cells at the bottom of the well.

Autologous control can be performed if required.