The identification test for unexpected antibodies using E.M.® Technology is based on the magnetization of red blood cells. The principle of this method uses the Indirect Antiglobulin Test (IAT) technique, in solid phase, combined with a magnetic field.
- The plasma or serum from the sample to be tested is incubated with the identification panel (11 group O magnetized red blood cells with known phenotypes) These red blood cells are sensitized in the presence of antibodies steered against the antigens being on their surface.
- Once exposed to a magnetic field, the magnetized red blood cells migrate to the bottom of the well.
- The monoclonal anti-IgG antiglobulin present in the wells of ScreenLys microplate reveals the presence of IgG type antibody fixed on the red blood cells. This in turn, leads to formation of a layer of red blood cells at the bottom of the well.